Part:BBa_M50436:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M50436

This protein can be used to speed up enterobactin synthesis slightly in E. coli. It works in tandem with entC (BBa_M50435), facilitating the transformation of chorismate to isochorismate.

User Reviews

UNIQ5c74851d865e5f2b-partinfo-00000000-QINU UNIQ5c74851d865e5f2b-partinfo-00000001-QINU

We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into E. coli, then plated the E. coli on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. See https://parts.igem.org/Part:BBa_M50437:Experience for a detailed assessment.